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Nirala Jacobi: I think one of the reasons everybody is so confused about it is that there really are no way of absolutely knowing whether or not somebody has this condition, unless they utilize this one lab in California called Gemelli Lab that does the Trio-Smart Test that looks for hydrogen sulfide as well

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Then, cDNAs were amplified using a 20-μL SYBR Green Supermix (Takara, Kyoto, Japan)